Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHB148
CRISPR-associated protein Csm2

Locus: 2:138875..139279
Synonym: TT1834
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 402
AA Length: 134
Mol. Weight: 15767
pI: 9.6
Absorption coefficient: 7350
Number of Methionine: 4

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC014148-41pET-11a 4available
PC014148-42pET-HisTEV 5not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC014148-41SeMet08/15/200610.4
PC014148-41SeMet07/09/20079
PC014148-42SeMet12/06/20065.1

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs11D01PD014148-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

Related Articles:
Takahata, Y. et al., (2012) Close proximity of phosphorylation sites to ligand in the phosphoproteome of the extreme thermophile Thermus thermophilus HB8 Proteomics 12 :  1414-30