Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA1892
excinuclease ABC subunit B, UvrB

Locus: 1:complement(1775514..1777511)
Synonym: TT0737
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 1995
AA Length: 665
Mol. Weight: 76169
pI: 5.4
Absorption coefficient: 59955
Number of Methionine: 9

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011892-41pET-11a 9available
PC011892-42pET-3aK42A9not available
PC011892-43pET-HisTEV 10not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011892-41Native05/06/200171.4
PC011892-41Native07/15/200535.9
PC011892-41Native03/10/200637.2
PC011892-41Native03/23/20079

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs10F02PD011892-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
1D2M09/25/1999
1C4O09/14/1999
the most similar PDB entry:

Related Articles:
Eryilmaz, J. et al., (2006) Structural insights into the cryptic DNA-dependent ATPase activity of UvrB J Mol Biol 357 :  62-72
Nakagawa, N. et al., (2001) [Structure and function of DNA repair enzyme UvrB from Thermus thermophilus HB8] Tanpakushitsu Kakusan Koso 46 :  968-75
Yamagata, A. et al., (2000) Interaction of UvrA and UvrB proteins with a fluorescent single-stranded DNA. Implication for slow conformational change upon interaction of UvrB with DNA J Biol Chem 275 :  13235-42
Nakagawa, N. et al., (1999) Crystal structure of Thermus thermophilus HB8 UvrB protein, a key enzyme of nucleotide excision repair J Biochem 126 :  986-90
Machius, M. et al., (1999) Crystal structure of the DNA nucleotide excision repair enzyme UvrB from Thermus thermophilus Proc Natl Acad Sci U S A 96 :  11717-22
Shibata, A. et al., (1999) Crystallization and preliminary X-ray diffraction studies of a DNA excision repair enzyme, UvrB, from Thermus thermophilus HB8 Acta Crystallogr D Biol Crystallogr 55 :  704-5
Nakagawa, N. et al., (1997) Domain structure of Thermus thermophilus UvrB protein. Similarity in domain structure to a helicase J Biol Chem 272 :  22703-13
Kato, R. et al., (1996) ATPase activity of UvrB protein form Thermus thermophilus HB8 and its interaction with DNA J Biol Chem 271 :  9612-8