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Thermus thermophilus HB8  TTHA1860
DNA polymerase III delta prime subunit

Locus: 1:complement(1743712..1744518)
Synonym: TT0745
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 804
AA Length: 268
Mol. Weight: 29344
pI: 7.4
Absorption coefficient: 24675
Number of Methionine: 2

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011860-41pET-11a 2available
PC011860-42pET-HisTEV 3not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011860-41Native11/07/200142
PC011860-41SeMet10/29/20049.1
PC011860-41Native10/24/20075.8

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs10C01PD011860-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

Related Articles:
Bullard, J. M. et al., (2002) DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase J Biol Chem 277 :  13401-8
Ruttimann, C. et al., (1985) DNA polymerases from the extremely thermophilic bacterium Thermus thermophilus HB-8 Eur J Biochem 149 :  41-6