Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA1823
hydrolase (phosphoglycolate phosphatase)

Locus: 1:1706239..1706865
Synonym: TT1328
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 624
AA Length: 208
Mol. Weight: 22651
pI: 7.8
Absorption coefficient: 17430
Number of Methionine: 1

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011823-41pET-11a 1available
PC011823-42pET-HisTEV 2not available
PC011823-43pET-11a 2not available
PC011823-44pET-11a 2not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011823-41Native03/30/200418
PC011823-41Native02/10/20051.5
PC011823-41Native10/11/200514.9
PC011823-41SeMet06/14/20065.9
PC011823-43SeMet01/30/2007 
PC011823-44SeMet02/02/20072.7
PC011823-42SeMet11/30/2006.2

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs09E11PD011823-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

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