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Thermus thermophilus HB8  TTHA1806
MutM protein (formamidopyrimidine-DNA glycosylase), Fpg

Locus: 1:1684475..1685278
Synonym: TT0730
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 801
AA Length: 267
Mol. Weight: 29919
pI: 10.6
Absorption coefficient: 10290
Number of Methionine: 2

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011806-41pET-11a  not available
PC011806-42pET-3a 2available
PC011806-43pET-HisTEV 2not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011806-42Native04/18/2005.2
PC011806-42Native05/27/200534.9
PC011806-42Native04/25/200630.2
PC011806-42Native05/15/20075.4

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs09D10PD011806-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
1EE801/31/2000
the most similar PDB entry:

Related Articles:
Sugahara, M. et al., (2000) Crystal structure of a repair enzyme of oxidatively damaged DNA, MutM (Fpg), from an extreme thermophile, Thermus thermophilus HB8 EMBO J 19 :  3857-69
Sugahara, M. et al., (2000) Crystallization and preliminary X-ray crystallographic studies of Thermus thermophilus HB8 MutM protein involved in repairs of oxidative DNA damage J Biochem 127 :  9-11