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Thermus thermophilus HB8  TTHA1757
putative N-acetyllysine deacetylase (lysine biosynthesis enzyme LysK)

Locus: 1:1645004..1646089
Synonym: TT0025
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 1083
AA Length: 361
Mol. Weight: 39599
pI: 7
Absorption coefficient: 30555
Number of Methionine: 7

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011757-41pET-11a 7not available
PC011757-42pET-11a 7available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011757-41Native07/19/20013.5
PC011757-41Native08/24/200118.6
PC011757-41Native05/22/20022.7
PC011757-42SeMet08/28/200614.5

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs09F07PD011757-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

Related Articles:
Nishida, H. and Nishiyama, M., (2012) Evolution of lysine biosynthesis in the phylum deinococcus-thermus Int J Evol Biol   :  745931
Horie, A. et al., (2009) Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus Nat Chem Biol 5 :  673-9