Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA1742
orotate phosphoribosyltransferase, pyrE

Locus: 1:complement(1631594..1632145)
Synonym: TT0120
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 549
AA Length: 183
Mol. Weight: 19452
pI: 6.9
Absorption coefficient: 4410
Number of Methionine: 3

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011742-41pET-11a 3available
PC011742-42pET-HisTEV 4not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011742-41Native05/20/20027.8
PC011742-41SeMet08/09/20064

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs09D06PD011742-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

Related Articles:
Mackwan, R. R. et al., (2008) The Rate and Character of Spontaneous Mutation in Thermus thermophilus Genetics 180 :  17-25