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Thermus thermophilus HB8  TTHA1697
30S ribosomal protein S12

Locus: 1:complement(1594930..1595337)
Synonym: TT0849
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 405
AA Length: 135
Mol. Weight: 14885
pI: 11.7
Absorption coefficient: 7350
Number of Methionine: 1

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011697-41pET-11a 1available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
4DR702/16/2012
3I9B07/10/2009
3I8H07/09/2009
2ZM604/14/2009
3F1E10/27/2008
2V4606/28/2007
2V4806/27/2007
2E5L05/15/2007
2J0207/31/2006
2HHH06/28/2006
2B9M10/12/2005
2B6409/30/2005
1GIX03/30/2001
1FJG08/08/2000
the most similar PDB entry:

Related Articles:
Demirci, H. et al., (2013) A structural basis for streptomycin-induced misreading of the genetic code Nat Commun 4 :  1355
Demeshkina, N. et al., (2012) A new understanding of the decoding principle on the ribosome Nature 484 :  256-9
Jenner, L. B. et al., (2010) Structural aspects of messenger RNA reading frame maintenance by the ribosome Nat Struct Mol Biol 17 :  555-60
Korostelev, A. et al., (2008) Crystal structure of a translation termination complex formed with release factor RF2 Proc Natl Acad Sci U S A 105 :  19684-9
Weixlbaumer, A. et al., (2007) Crystal structure of the ribosome recycling factor bound to the ribosome Nat Struct Mol Biol 14 :  733-7
Carr, J. F. et al., (2006) Effects of streptomycin resistance mutations on posttranslational modification of ribosomal protein S12 J Bacteriol 188 :  2020-3
Carr, J. F. et al., (2005) Severity of the streptomycin resistance and streptomycin dependence phenotypes of ribosomal protein S12 of Thermus thermophilus depends on the identity of highly conserved amino acid residues J Bacteriol 187 :  3548-50
Spiridonova, V. A. et al., (1999) A study of the thermophilic ribosomal protein S7 binding to the truncated S12-S7 intercistronic region provides more insight into the mechanism of regulation of the str operon of E. coli(1) FEBS Lett 460 :  353-6
Yakhnin, A. V. et al., (1990) Nucleotide sequence of the Thermus thermophilus HB8 rps12 and rps7 genes coding for the ribosomal proteins S12 and S7 Nucleic Acids Res 18 :  3659