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Thermus thermophilus HB8  TTHA1645
MutS2 protein

Locus: 1:complement(1559701..1561935)
Synonym: TT0723
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 2232
AA Length: 744
Mol. Weight: 82527
pI: 7.1
Absorption coefficient: 21945
Number of Methionine: 11

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011645-41  11available
PC011645-42pET-HisTEV 12not available
PC011645-43pET-15b 3not available
PC011645-44pET-15bE677A3not available
PC011645-45pET-11a 1not available
PC011645-46pET-11a 1not available
PC011645-47pET-11a 9not available
PC011645-48pET-11a 9not available
PC011645-49pET-11a 11not available
PC011645-50pET-11a 9not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011645-42Native11/22/200612.5
PC011645-42SeMet10/16/20071.3
PC011645-46Native02/29/20086.3
PC011645-46SeMet05/26/20084
PC011645-42Native02/13/20098.4
PC011645-43SeMet04/16/2009.4
PC011645-44SeMet07/09/2009 

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs09B01PD011645-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
2ZQE08/08/2008
the most similar PDB entry:

Related Articles:
Fukui, K. et al., (2008) Crystal structure of MutS2 endonuclease domain and the mechanism of homologous recombination suppression J Biol Chem 283 :  33417-27
Fukui, K. et al., (2007) Analysis of a nuclease activity of catalytic domain of Thermus thermophilus MutS2 by high-accuracy mass spectrometry Nucleic Acids Res 35 :  e100
Fukui, K. et al., (2007) Nuclease activity of the MutS homologue MutS2 from Thermus thermophilus is confined to the Smr domain Nucleic Acids Res 35 :  850-60
Fukui, K. et al., (2004) Thermus thermophilus MutS2, a MutS paralogue, possesses an endonuclease activity promoted by MutL J Biochem 135 :  375-84