Whole Cell Project Database -Target Detail-

Show All

List View


Thermus thermophilus HB8  TTHA1435
purine nucleoside phosphorylase, TtPNPI

Locus: 1:complement(1362832..1363539)
Synonym: TT0127
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 705
AA Length: 235
Mol. Weight: 25418
pI: 6.5
Absorption coefficient: 34860
Number of Methionine: 5

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011435-41pET-11a 5available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs07H09PD011435-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
1ODI02/19/2003
1ODJ02/19/2003
1ODK02/19/2003
1ODL02/19/2003
the most similar PDB entry:

Related Articles:
Almendros, M. et al., (2012) Nucleoside Phosphorylases from Thermus thermophilus active in the synthesis of nucleoside analogues Appl Environ Microbiol 78 :  3128-35
Tahirov, T. H. et al., (2004) Crystal structure of purine nucleoside phosphorylase from Thermus thermophilus J Mol Biol 337 :  1149-60