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Thermus thermophilus HB8  TTHA1324
DNA mismatch repair protein MutS

Locus: 1:complement(1261786..1264245)
Synonym: TT0734
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 2457
AA Length: 819
Mol. Weight: 91393
pI: 5.6
Absorption coefficient: 51240
Number of Methionine: 14

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011324-41pET-3a 14available
PC011324-42pET-HisTEV 15not available
PC011324-43pET-11aC-terminal domain-deleted mutant of T. thermophilus MutS.13not available
PC011324-44pET-15btn 14not available
PC011324-45pET-15btn3 14not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011324-41Native08/08/20073
PC011324-41Native10/31/20072
PC011324-42Native12/01/20067.5
PC011324-41Native03/19/20092.6

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs07F04PD011324-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

Related Articles:
Stanislawska-Sachadyn, A. et al., (2006) The construction of bifunctional fusion proteins consisting of MutS and GFP J Biotechnol 121 :  134-43
Stanislawska-Sachadyn, A. and Sachadyn, P., (2005) MutS as a tool for mutation detection Acta Biochim Pol 52 :  575-83
Stanislawska-Sachadyn, A. et al., (2005) Preliminary studies on DNA retardation by MutS applied to the detection of point mutations in clinical samples Mutat Res 570 :  97-103
Fukui, K. et al., (2004) Thermus thermophilus MutS2, a MutS paralogue, possesses an endonuclease activity promoted by MutL J Biochem 135 :  375-84
Stanislawska-Sachadyn, A. et al., (2003) Construction and purification of his6-Thermus thermophilus MutS protein Protein Expr Purif 28 :  69-77
Kato, R. et al., (2001) Direct observation of three conformations of MutS protein regulated by adenine nucleotides J Mol Biol 309 :  227-38
Tachiki, H. et al., (2000) DNA binding and protein-protein interaction sites in MutS, a mismatched DNA recognition protein from Thermus thermophilus HB8 J Biol Chem 275 :  40703-9
Tachiki, H. et al., (1998) Domain organization and functional analysis of Thermus thermophilus MutS protein Nucleic Acids Res 26 :  4153-9