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Thermus thermophilus HB8  TTHA1323
DNA mismatch repair protein MutL

Locus: 1:complement(1260152..1261789)
Synonym: TT0735
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAA
DNA Length: 1635
AA Length: 545
Mol. Weight: 60110
pI: 9
Absorption coefficient: 32025
Number of Methionine: 2

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011323-41pET-11a 2available
PC011323-42pColdIV 2not available
PC011323-43pET-HisTEV 3not available
PC011323-44pMAL-cRI 9not available
PC011323-45pMAL-C2XD364N2not available
PC011323-46pET-11aD364N2not available
PC011323-47pET-11aC496A2not available
PC011323-48pET-11a 2not available
PC011323-49pET-11a 1not available
PC011323-50pET-11a 2not available
PC011323-51pET-11a 1not available
PC011323-52pMAL-C2XC496A2not available
PC011323-53pMAL-cRI 8not available
PC011323-54pET-HisTEV  not available
PC011323-55pET-11aE28A2not available
PC011323-56pET-11aD57A2not available
PC011323-57pMAL-cRI 9not available
PC011323-58pMAL-cRI 9not available
PC011323-59pMAL-cRI 9not available
PC011323-60pMAL-cRI 9not available
PC011323-61pMAL-cRI 9not available
PC011323-62pET-HisTEVThe C-terminal domain of MutL.3not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011323-41Native08/07/20075.8
PC011323-41Native10/04/20071.8
PC011323-46Native11/09/2007.3
PC011323-47Native11/14/2007.7
PC011323-47Native11/20/2007.1
PC011323-41Native11/21/2007.7
PC011323-47Native11/27/2007.1
PC011323-41Native11/28/20071.8
PC011323-47Native01/22/20083
PC011323-41Native02/07/20084.6
PC011323-41Native04/07/200819.3
PC011323-47Native04/12/2008 
PC011323-41Native08/01/2008 
PC011323-41Native08/01/20081.7
PC011323-44Native10/14/20083.6
PC011323-44Native11/11/2008 
PC011323-44Native11/11/200875.1
PC011323-41Native11/25/2008 
PC011323-48Native12/01/20081.5

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs07E04PD011323-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

Related Articles:
Fukui, K. et al., (2008) Bound Nucleotide Controls the Endonuclease Activity of Mismatch Repair Enzyme MutL J Biol Chem 283 :  12136-45