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Thermus thermophilus HB8  TTHA1279
V-type ATP synthase, subunit C (VAPC-THERM)

Locus: 1:complement(1220798..1221112)
Synonym: TT0380
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 312
AA Length: 104
Mol. Weight: 11674
pI: 7
Absorption coefficient: 2940
Number of Methionine: 2

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011279-41pET-11a 3available
PC011279-42pET-HisTEV 4not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011279-41Native08/28/20024.6
PC011279-41Native12/10/20033.2

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
3K5B10/07/2009
the most similar PDB entry:

Related Articles:
Lee, L. K. et al., (2010) The structure of the peripheral stalk of Thermus thermophilus H(+)-ATPase/synthase Nat Struct Mol Biol 17 :  373-8
Lau, W. C. and Rubinstein, J. L., (2010) Structure of intact Thermus thermophilus V-ATPase by cryo-EM reveals organization of the membrane-bound VO motor Proc Natl Acad Sci U S A 107 :  1367-72
Boesen, T. and Nissen, P., (2009) V for victory--a V1-ATPase structure revealed EMBO reports 10 :  1211-2
Nakano, M. et al., (2008) ATP hydrolysis and synthesis of a rotary motor V-ATPase from thermus thermophilus The Journal of biological chemistry 283 :  20789-20796
Iwata, M. et al., (2004) Crystal structure of a central stalk subunit C and reversible association/dissociation of vacuole-type ATPase Proc Natl Acad Sci U S A 101 :  59-64
Yokoyama, K. et al., (2003) Subunit arrangement in V-ATPase from Thermus thermophilus J Biol Chem 278 :  42686-91