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Thermus thermophilus HB8  TTHA1167
single-stranded DNA specific exonuclease RecJ

Locus: 1:1109423..1111423
Synonym: TT0716
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 1998
AA Length: 666
Mol. Weight: 72874
pI: 6.3
Absorption coefficient: 89880
Number of Methionine: 6

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011167-41pET-11a 6available
PC011167-42pET-HisTEV 7not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011167-41Native06/11/20074.6
PC011167-41SeMet03/21/20083.7
PC011167-41Native04/03/2008.9
PC011167-41SeMet04/24/20083.9

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs06A08PD011167-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
2ZXO01/05/2009
2ZXP01/05/2009
2ZXR01/05/2009
1IR609/11/2001
the most similar PDB entry:

Related Articles:
Wakamatsu, T. et al., (2010) Structure of RecJ exonuclease defines its specificity for single-stranded DNA J Biol Chem 285 :  9762-9