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Thermus thermophilus HB8  TTHA1150
DNA polymerase beta family (X family)

Locus: 1:complement(1095294..1097021)
Synonym: TT0748
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 1725
AA Length: 575
Mol. Weight: 64118
pI: 6.7
Absorption coefficient: 66885
Number of Methionine: 9

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011150-41pET-11a 9available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011150-41Native06/13/20011.4
PC011150-41Native09/03/20015.5
PC011150-41Native05/27/200316.2
PC011150-41SeMet10/22/20044.5
PC011150-41Native04/05/200515.1
PC011150-41Native08/10/20058.1
PC011150-41SeMet08/17/200518.5
PC011150-41Native07/04/200740.8

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs06H06PD011150-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
3AU002/11/2011
3AU201/28/2011
3AU601/28/2011
the most similar PDB entry:

Related Articles:
Nakane, S. et al., (2012) The Structural Basis of the Kinetic Mechanism of a Gap-Filling X-Family DNA Polymerase That Binds Mg(2+)-dNTP Before Binding to DNA J Mol Biol 417 :  179-96
Nakane, S. et al., (2009) Characterization of DNA polymerase X from Thermus thermophilus HB8 reveals the POLXc and PHP domains are both required for 3'-5' exonuclease activity Nucleic Acids Res 37 :  2037-52
Ruttimann, C. et al., (1985) DNA polymerases from the extremely thermophilic bacterium Thermus thermophilus HB-8 Eur J Biochem 149 :  41-6