Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA1097
DNA ligase [NAD+]

Locus: 1:complement(1042879..1044909)
Synonym: TT0727
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAA
DNA Length: 2028
AA Length: 676
Mol. Weight: 76915
pI: 7.1
Absorption coefficient: 59745
Number of Methionine: 7

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011097-41pET-3a 7not available
PC011097-42pET-HisTEV 8not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011097-41Native09/17/20013
PC011097-41Native05/30/200533.2
PC011097-41Native05/21/20071.2

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs06H03PD011097-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
1L7B03/14/2002
the most similar PDB entry:

Related Articles:
Lauer, G. et al., (1991) Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase J Bacteriol 173 :  5047-53