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Thermus thermophilus HB8  TTHA1054
DNA polymerase I

Locus: 1:996840..999344
Synonym: TT0742
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 2502
AA Length: 834
Mol. Weight: 94018
pI: 6.6
Absorption coefficient: 100170
Number of Methionine: 16

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011054-41pET-11a 16available
PC011054-42pET-HisTEV 17not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011054-41Native03/04/20047
PC011054-41Native06/10/20057.1
PC011054-41Native05/24/20079.2
PC011054-42Native05/12/20100
PC011054-42Native11/12/201017.5

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs06E01PD011054-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

Related Articles:
Eilert, K. D. and Foran, D. R., (2009) Polymerase Resistance to Polymerase Chain Reaction Inhibitors in Bone* J Forensic Sci 54 :  1001-7
Belousova, E. A. et al., (2006) Thermostable DNA polymerases can perform translesion synthesis using 8-oxoguanine and tetrahydrofuran-containing DNA templates Biochim Biophys Acta 1764 :  97-104
Moreno, R. et al., (2005) High-level overproduction of His-tagged Tth DNA polymerase in Thermus thermophilus Appl Environ Microbiol 71 :  591-3
Rechkunova, N. I. et al., (2000) Thermostable DNA polymerase from Thermus thermophilus B35: influence of divalent metal ions on the interaction with deoxynucleoside triphosphates Biochemistry (Mosc) 65 :  609-14
Rechkunova, N. I. et al., (1998) Thermostable DNA-polymerase from Thermus thermophilus B35: isolation and characterization of some properties Biochemistry (Mosc) 63 :  1266-70
Ogata, N. and Miura, T., (1998) Creation of genetic information by DNA polymerase of the thermophilic bacterium Thermus thermophilus Nucleic Acids Res 26 :  4657-61
Kim, J. S. et al., (1998) Top DNA polymerase from Thermus thermophilus HB27: gene cloning, sequence determination, and physicochemical properties Mol Cells 8 :  157-61
Auer, T. et al., (1995) Properties of the 5'-->3' exonuclease/ribonuclease H activity of Thermus thermophilus DNA polymerase Biochemistry 34 :  4994-5002
Chang, H.(1994) In situ transcription with Tth DNA polymerase and fluorescent nucleotides J Immunol Methods 176 :  235-43
Kuz'min, A. N. et al., (1992) [The use of DNA polymerase from Thermus thermophilus] Vopr Virusol 37 :  94-5
Gaensslen, R. E. et al., (1992) A polymerase chain reaction (PCR) method for sex and species determination with novel controls for deoxyribonucleic acid (DNA) template length J Forensic Sci 37 :  6-20
Bechtereva, T. A. et al., (1989) DNA sequencing with thermostable Tet DNA polymerase from Thermus thermophilus Nucleic Acids Res 17 :  10507
Ruttimann, C. et al., (1985) DNA polymerases from the extremely thermophilic bacterium Thermus thermophilus HB-8 Eur J Biochem 149 :  41-6