Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0889
transcription-repair coupling factor

Locus: 1:845828..848764
Synonym: TT0739
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 2934
AA Length: 978
Mol. Weight: 109753
pI: 6.9
Absorption coefficient: 96180
Number of Methionine: 6

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010889-41pET-11a 6available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010889-41Native12/02/2002.8
PC010889-41Native12/16/20024.3
PC010889-41SeMet01/21/20031.4
PC010889-41Native06/20/200427.3
PC010889-41Native10/19/200532

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs05C05PD010889-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

Related Articles:
Westblade, L. F. et al., (2010) Structural basis for the bacterial transcription-repair coupling factor/RNA polymerase interaction Nucleic Acids Res 38 :  8357-8369