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Thermus thermophilus HB8  TTHA0875
seryl-tRNA synthetase

Locus: 1:complement(833881..835146)
Synonym: TT0872
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 1263
AA Length: 421
Mol. Weight: 47793
pI: 6.3
Absorption coefficient: 58170
Number of Methionine: 9

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010875-41pET-11a 9available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
3ERR10/03/2008
1SES02/21/1994
1SET02/21/1994
1SRY08/10/1993
the most similar PDB entry:

Related Articles:
McClendon, C. L. et al., (2006) Fidelity of seryl-tRNA synthetase to binding of natural amino acids from HierDock first principles computations Protein Eng Des Sel 19 :  195-203
Lenhard, B. et al., (1997) Defining the active site of yeast seryl-tRNA synthetase. Mutations in motif 2 loop residues affect tRNA-dependent amino acid recognition J Biol Chem 272 :  1136-41
Cusack, S. et al., (1996) The crystal structure of the ternary complex of T.thermophilus seryl-tRNA synthetase with tRNA(Ser) and a seryl-adenylate analogue reveals a conformational switch in the active site EMBO J 15 :  2834-42
El-Kettani, M. A. and Smith, J. C., (1996) Pathways for conformational change in seryl-tRNA synthetase from Thermus thermophilus C R Acad Sci III 319 :  161-9
Belrhali, H. et al., (1995) The structural basis for seryl-adenylate and Ap4A synthesis by seryl-tRNA synthetase Structure 3 :  341-52
Belrhali, H. et al., (1994) Crystal structures at 2.5 angstrom resolution of seryl-tRNA synthetase complexed with two analogs of seryl adenylate Science 263 :  1432-6
Biou, V. et al., (1994) The 2.9 A crystal structure of T. thermophilus seryl-tRNA synthetase complexed with tRNA(Ser) Science 263 :  1404-10
Fujinaga, M. et al., (1993) Refined crystal structure of the seryl-tRNA synthetase from Thermus thermophilus at 2.5 A resolution J Mol Biol 234 :  222-33
Yaremchuk, A. D. et al., (1992) A new crystal form of the complex between seryl-tRNA synthetase and tRNA(Ser) from Thermus thermophilus that diffracts to 2.8 A resolution FEBS Lett 310 :  157-61
Yaremchuk, A. D. et al., (1992) Crystallization of the seryl-tRNA synthetase-tRNA(Ser) complex from Thermus thermophilus J Mol Biol 224 :  519-22
Garber, M. B. et al., (1990) Crystals of seryl-tRNA synthetase from Thermus thermophilus. Preliminary crystallographic data J Mol Biol 213 :  631-2