Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0834
endonuclease IV

Locus: 1:complement(799876..800688)
Synonym: TT0715d
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAA
DNA Length: 810
AA Length: 270
Mol. Weight: 29089
pI: 6.3
Absorption coefficient: 36225
Number of Methionine: 1

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010834-41pET-15b 2available
PC010834-42pET-11a  not available
PC010834-43pET-HisTEV 2not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010834-41Native09/12/2007121.1

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs05D01PD010834-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
3AAM07/13/2011
the most similar PDB entry:

Related Articles:
Asano, R. et al., (2011) An additional C-terminal loop in endonuclease IV, an apurinic/apyrimidinic endonuclease, controls binding affinity to DNA Acta Crystallogr D Biol Crystallogr 67 :  149-55
Schomacher, L. et al., (2010) Helix-hairpin-helix protein MJ1434 from Methanocaldococcus jannaschii and EndoIV homologue TTC0482 from Thermus thermophilus HB27 do not process DNA uracil residues Nucleic Acids Res 38 :  5119-29