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Thermus thermophilus HB8  TTHA0812
phosphoribosylamine-glycine ligase, PurD

Locus: 1:774831..776084
Synonym: TT0101
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 1251
AA Length: 417
Mol. Weight: 44468
pI: 5.2
Absorption coefficient: 40740
Number of Methionine: 8

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010812-41pET-11a 8available
PC010812-42pET-11a  not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010812-41Native05/17/200154.6
PC010812-41Native06/06/200164
PC010812-41SeMet07/30/200239.6
PC010812-41Native06/29/2007100.9
PC010812-41Native07/26/200798.5

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs04F11PD010812-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
2IP410/11/2006
the most similar PDB entry:

Related Articles:
Sampei, G. I. et al., (2010) Crystal Structures of Glycinamide Ribonucleotide Synthetase, PurD, from Thermophilic Eubacteria J Biochem 148 :  429-38