Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0788
DNA polymerase III, delta subunit

Locus: 1:complement(754477..755355)
Synonym: TT1349
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 876
AA Length: 292
Mol. Weight: 32431
pI: 8.9
Absorption coefficient: 21735
Number of Methionine: 3

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010788-41pET-11aF220L3not available
PC010788-42pET-11a 3available
PC010788-43pET-HisTEV 4not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010788-42SeMet10/29/20049.1
PC010788-42Native10/24/20075.8

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs04E09PD010788-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

Related Articles:
Bullard, J. M. et al., (2002) DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase J Biol Chem 277 :  13401-8
Ikeda, K. et al., (1995) A non-radioactive DNA sequencing method using biotinylated dideoxynucleoside triphosphates and delta Tth DNA polymerase DNA Res 2 :  225-7
Ruttimann, C. et al., (1985) DNA polymerases from the extremely thermophilic bacterium Thermus thermophilus HB-8 Eur J Biochem 149 :  41-6