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Thermus thermophilus HB8  TTHA0656
ribosomal protein L11 methyltransferase

Locus: 1:622962..623726
Synonym: TT0836
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 762
AA Length: 254
Mol. Weight: 27634
pI: 4.8
Absorption coefficient: 59325
Number of Methionine: 4

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010656-41pET-11aP225S4not available
PC010656-42pET-11a 4available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010656-41Native09/21/200148.4
PC010656-41SeMet03/06/200215.1
PC010656-41Native09/03/200278.5

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
 PD010656-01 HTK  
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
3CJU03/13/2008
3CJT03/13/2008
3CJS03/13/2008
3CJR03/13/2008
3CJQ03/13/2008
2zbp10/26/2007
2zbq10/26/2007
2zbr10/26/2007
2NXN11/17/2006
2NXC11/17/2006
2NXE11/17/2006
2NXJ11/17/2006
1UFK05/31/2003
the most similar PDB entry:

Related Articles:
Demirci, H. et al., (2008) Multiple-site trimethylation of ribosomal protein L11 by the PrmA methyltransferase Structure 16 :  1059-66
Demirci, H. et al., (2007) Recognition of ribosomal protein L11 by the protein trimethyltransferase PrmA EMBO J 26 :  567-77
Cameron, D. M. et al., (2004) Thermus thermophilus L11 methyltransferase, PrmA, is dispensable for growth and preferentially modifies free ribosomal protein L11 prior to ribosome assembly J Bacteriol 186 :  5819-25
Kaminishi, T. et al., (2003) Crystallization and preliminary X-ray diffraction analysis of ribosomal protein L11 methyltransferase from Thermus thermophilus HB8 Acta Crystallogr D Biol Crystallogr 59 :  930-2