Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0609
tRNA (adenine-N(1)-)-methyltransferase

Locus: 1:complement(577206..577973)
Synonym: TT1321
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 765
AA Length: 255
Mol. Weight: 28572
pI: 8.1
Absorption coefficient: 43470
Number of Methionine: 5

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010609-41pET-11a 5available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010609-41 03/31/2000 

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs03A12PD010609-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
2PWY05/14/2007
the most similar PDB entry:

Related Articles:
Barraud, P. et al., (2008) Crystal structure of Thermus thermophilus tRNA m1A58 methyltransferase and biophysical characterization of its interaction with tRNA J Mol Biol 377 :  535-50