Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0533
ribosomal RNA small subunit methyltransferase (16S rRNA N2 G1207 methyltransferase, RsmC)

Locus: 1:complement(495541..496668)
Synonym: TT1444
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 1125
AA Length: 375
Mol. Weight: 40661
pI: 9.5
Absorption coefficient: 34965
Number of Methionine: 4

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010533-41pET-11a 4available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010533-41SeMet01/13/2004.4
PC010533-41Native06/14/200410.1
PC010533-41Native02/14/20053.9
PC010533-41SeMet02/16/200526.7

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs03A08PD010533-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
3dmf07/01/2008
3dmg07/01/2008
3dmh07/01/2008
the most similar PDB entry:

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