Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0478
trehalose synthase

Locus: 1:complement(446574..449471)
Synonym: TT0528
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 2895
AA Length: 965
Mol. Weight: 110607
pI: 6
Absorption coefficient: 182910
Number of Methionine: 16

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010478-41pET-11a 16available
PC010478-42pET-21bI238T,A431T,F518L,F534L,E560G16not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010478-41Native02/15/200524.4
PC010478-41Native09/15/20053.2
PC010478-41SeMet10/19/20051.8

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

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