Whole Cell Project Database -Target Detail-

Show All

List View


Thermus thermophilus HB8  TTHA0327
5,10-methylenetetrahydrofolate reductase

Locus: 1:complement(314192..315082)
Synonym: TT0187
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAA
DNA Length: 888
AA Length: 296
Mol. Weight: 33006
pI: 9.3
Absorption coefficient: 16065
Number of Methionine: 4

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010327-41pET-11aF95X4not available
PC010327-42pET-11a 4available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010327-41Native06/29/2001136.3
PC010327-41Native03/31/2000 

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
3APY10/21/2010
3APT10/20/2010
1V9301/20/2004
the most similar PDB entry:

Related Articles:
Igari, S. et al., (2011) Properties and Crystal Structure of Methylenetetrahydrofolate Reductase from Thermus thermophilus HB8 PloS one 6 :  e23716