Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0244
single-stranded DNA binding protein

Locus: 1:complement(235462..236253)
Synonym: TT0768
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 789
AA Length: 263
Mol. Weight: 29886
pI: 4.9
Absorption coefficient: 40530
Number of Methionine: 4

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010244-41pET-11aF255P4not available
PC010244-42pET-11a 4available
PC010244-43pET-HisTEV 5not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010244-41Native04/01/2001 
PC010244-42SeMet09/17/20041.7
PC010244-42Native03/25/20054
PC010244-42Native06/19/2007 

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs02H02PD010244-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
2CWA06/17/2005
the most similar PDB entry:

Related Articles:
Shi, H. et al., (2013) Systematic Functional Comparative Analysis of Four Single-Stranded DNA-Binding Proteins and Their Affection on Viral RNA Metabolism PloS one 8 :  e55076