Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0210
50S ribosomal protein L12

Locus: 1:206642..207019
Synonym: TT0809
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAA
DNA Length: 375
AA Length: 125
Mol. Weight: 13069
pI: 4.8
Absorption coefficient: 5775
Number of Methionine: 1

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010210-41unknown  not available
PC010210-42pET-21a(+) 1not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
3I8I07/09/2009
2J0307/31/2006
the most similar PDB entry:

Related Articles:
Jenner, L. B. et al., (2010) Structural aspects of messenger RNA reading frame maintenance by the ribosome Nat Struct Mol Biol 17 :  555-60
Nomura, T. et al., (2008) Biochemical evidence for the heptameric complex L10(L12)6 in the Thermus thermophilus ribosome: in vitro analysis of its molecular assembly and functional properties J Biochem 144 :  665-673
Selmer, M. et al., (2006) Structure of the 70S ribosome complexed with mRNA and tRNA Science 313 :  1935-42