Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0188
nucleoside diphosphate kinase

Locus: 1:184971..185384
Synonym: TT0088
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAA
DNA Length: 411
AA Length: 137
Mol. Weight: 15346
pI: 7.9
Absorption coefficient: 1470
Number of Methionine: 6

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010188-41pET-11a 6available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010188-41Native09/19/20012.6
PC010188-41Native06/14/200219.6
PC010188-41Native10/18/2002 
PC010188-41Native02/06/2003 
PC010188-41Native08/16/2006 

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs01A12PD010188-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
1WKK06/01/2004
1WKL06/01/2004
1WKJ05/31/2004
the most similar PDB entry:

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