Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0180
DNA polymerase III alpha subunit

Locus: 1:complement(171503..177706)
Synonym: TT0743
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 6201
AA Length: 2067
Mol. Weight: 230039
pI: 7.3
Absorption coefficient: 215460
Number of Methionine: 34

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs01D11PD010180-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

Related Articles:
Bullard, J. M. et al., (2002) DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase J Biol Chem 277 :  13401-8
Ruttimann, C. et al., (1985) DNA polymerases from the extremely thermophilic bacterium Thermus thermophilus HB-8 Eur J Biochem 149 :  41-6