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Thermus thermophilus HB8  TTHA0086
NADH-quinone oxidoreductase chain 5

Locus: 1:84664..85287
Synonym: TT0440
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 621
AA Length: 207
Mol. Weight: 23862
pI: 5.8
Absorption coefficient: 39270
Number of Methionine: 3

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010086-41pET-11aR11W3not available
PC010086-42pET-11a 3available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010086-41Native06/26/20016.3
PC010086-41Native08/09/20011.3
PC010086-41Native08/28/20014.5
PC010086-41SeMet12/10/20013.2
PC010086-42Native10/28/20052.8

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
3M9S03/22/2010
3IAS07/14/2009
3IAM07/14/2009
3I9V07/13/2009
2FUG01/26/2006
the most similar PDB entry:

Related Articles:
Efremov, R. G. et al., (2010) The architecture of respiratory complex I Nature 465 :  441-5
Roessler, M. M. et al., (2010) Direct assignment of EPR spectra to structurally defined iron-sulfur clusters in complex I by double electron-electron resonance Proc Natl Acad Sci U S A 107 :  1930-5
Berrisford, J. M. and Sazanov, L. A., (2009) Structural basis for the mechanism of respiratory complex I J Biol Chem 284 :  29773-83
Sazanov, L. A. and Hinchliffe, P., (2006) Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus Science 311 :  1430-6