Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0074
DNA topoisomerase IA

Locus: 1:complement(68753..71245)
Synonym: TT0764
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TGA
DNA Length: 2490
AA Length: 830
Mol. Weight: 93455
pI: 8.8
Absorption coefficient: 97545
Number of Methionine: 8

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010074-41pET-11a 8available
PC010074-42pET-HisTEV 9not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010074-41Native01/26/20054.7
PC010074-41Native11/09/20054.3

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs01A05PD010074-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

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