Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0061
hypothetical protein

Locus: 1:complement(58225..58545)
Synonym: TT1907
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 318
AA Length: 106
Mol. Weight: 11835
pI: 4.1
Absorption coefficient: 20265
Number of Methionine: 1

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010061-41pET-11a 1available
PC010061-42pET-11aL84M2not available
PC010061-43pET-11aL11M2not available
PC010061-44pET-HisTEV 2not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010061-41Native12/02/200310.2
PC010061-41Native07/16/200417.8
PC010061-41Native07/25/200521.4
PC010061-41Native02/02/20068.2
PC010061-42SeMet05/16/200614.1
PC010061-43SeMet05/16/20067.4

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs01C04PD010061-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
2EI504/02/2007
2EBG02/08/2007
2EBE02/08/2007
the most similar PDB entry:

Related Articles:
Tanaka, T. et al., (2010) Crystal structure of TTHA0061, an uncharacterized protein from Thermus thermophilus HB8, reveals a novel fold Biochem Biophys Res Commun 400 :  258-64